Drosophila Protein interaction Map (DPiM)

Drosophila Protein interaction Map (DPiM)

Proteins perform essential cellular functions as part of protein complexes, often in conjunction with RNA, DNA, metabolites and other small molecules. The genome encodes thousands of proteins but not all of them are expressed in every cell type; and expressed proteins are not active at all times. Such diversity of protein expression and function accounts for the level of biological intricacy se...

Drosophila Protein interaction Map (DPiM): A paradigm for metazoan protein complex interactions

A protein interaction map of Drosophila melanogaster.

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybr...

Towards an Integrated Protein-protein Interaction Map

As protein-protein interaction plays a major role in most cellular processes, identifying the full repertoire of interacting protein pairs in the cell is of great importance. This challenge has been addressed both experimentally and computationally. Large-scale experimental studies provide data sets of interacting proteins. These data sets, while only partial and noisy, allow us to characterize...

A Protein Complex Network of Drosophila melanogaster

Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define indiv...